Microscopy fluorescent: izici ucwaningo

Igama elithi "isibonakhulu" elisuka ngesiGreki. Enguqulweni ke kusho ukufunda ngezinto nge high-ukucacisa amathuluzi. . Eminyakeni yamuva sekwande kakhulu Fluorescent kanye electron microscopy.

isinqumo

Ngaphansi kwalo, njengoba umthetho, ukuqonda ibanga okungenani lapho kungenzeka izinto ngokucacile akachazeki. On amandla isinqumo imishini degree sokungena kuzoncika emhlabeni ezincane, ungabona ubukhulu isici. With ukwanda okukhulu umngcele izindawo ungakwazi uhlangane. Ngakho, kukhona imikhawulo ethile, okungafanele indlela izici alusho lutho.

Indlela fluorescence microscopy: indlela

Lapho ukuguqulwa amandla olubamba izidakamizwa ku emisebeni ebonakalayo kwenzeka kukhanya. Ibizwa ngokuthi luminescence. Lesi simo esiyingqayizivele sibangelwa kungenxa yokuthi ezinye izinto ngaphansi kwethonya ukukhanya uqale bungakhiphi misebe kusuka kwenye (ngokuvamile ezinkulu) wavelength. Futhi, ezinye izinto ezimweni ezivamile ukukhanyisa ecacisiwe umbala bedle zokukhanya oku-ultraviolet kushintsha umbala waso.

ngokucacile

Into ukuthi ingabonakali ngaphansi UV ukukhanya, engase ikhiphe ayebuthaka elikhanyayo, uma iphathwa nge isakhi esithile esikhethekile. Ngo izakhi ke imibala ezahlukene ukukhanya ebumnyameni. umfutho ekhazimulayo ihlukile, kodwa njengoba umthetho, kuyinto encane. Kule ndaba, fluorescence microscopy liphumelele ikamelo mnyama. Uma usebenzisa lolu hlobo locwaningo kungabonakala ukukhanya into, yena obusuka. Ukwakheka kwamakhemikhali kwezicubu, amaseli sizothinta izinga lesifundo. Fluorescence microscopy ubhekwa othile ngokwezinga cwaningo histochemical.

ngezigaba

может быть первичной или вторичной. Fluorescence microscopy kungaba samabanga aphansi noma. Kulo mBhalo, into icutshungulwe nge compounds ekhethekile akunika luminescence. основывается на собственной способности исследуемого элемента излучать свет. microscopy Primary Fluorescent edzimelele ekghonweni yabo isici ezishisa kakhulu zikhipha izinhlayiyana zokukhanya.

imishini

Fluorescence microscopy wenziwa usebenzisa ezihlukahlukene amadivaysi. I lwenkanyiso ukhonza njengomdala umnyombo isici. Inama UV isibani. Ngaphezu kwalokho, idivayisi isebenzisa sihlungi. Kwezinye amathuluzi kukhona impela ukucupha ezimbalwa ezahlukahlukene. Kuye ngokuthi yiziphi umbala isetshenziselwa luminescence excitation - ultraviolet noma okwesibhakabhaka, phakathi umthombo wokukhanya kanye into ngaphansi kophenyo ibekwe yesihlungi ezifanele. Njengoba luminescence isici ezincane bazisa buthaka excitation lulula, bayobanjwa kuphela ngaphansi kombandela owodwa. Inala ray esukela umthombo unqunywe isihlungi ophuzi. It ibekwe phezu eyepiece yomculo. I evelele kunazo umphumela luminescence kuyokwenzeka lapho lemifanekiso is ancamuka imisebe aphumayo ukukhanya emthonjeni.

Kuyini ukufakwa ekukhanyeni ebonakalayo? Kuyo kuxhopha umthombo wokukhanya kanye ngesibonakhulu begazi. Phakathi esibukweni iyunithi nelangabi ukukhwezwa blue-violet zokuhlunga. Lokhu kungaba FS-1, UFS-3 nokunye. isihlungi Yellow esifanelana phezu eyepiece ka-microscope. Ngosizo lwabo, into falling blue-violet ukukhanya. Yena luminescence ujabule. Kodwa lokhu kukhanya kungaphazamisa isibonisi ukukhanya. Ngakho-ke, endleleni eya iso lapho zigundiwe isihlungi ophuzi. Ukufakwa ukukhanyisa eyenziwe Koehler indlela, ngaphandle nje kwento eyodwa. Diaphragm epholisayo kufanele kube usuvulekile. Esifundweni kubalulekile ukusebenzisa non-Fluorescent uwoyela ngokucwiliswa. Ukuze wehlise ekushayweni eziwumgogodla sengezwe nalokho nitrobenzene (2-10 sehla / 1 g).

Fluorescent microscopy: izicelo ku-microbiology

Izinzuzo lolu hlobo ucwaningo yilezi:

1. Kungenzeka ukuthola umbala izithombe.
2. yokugqama self-ukhanyisa izakhi on isizinda black.
3. Ukuthola kanye kwasendaweni kohlobo oluthile amagciwane namagciwane.
4. I kungenzeka ukutadisha esobala futhi opaque eziphilayo eziphilayo.
5. Lolu cwaningo zokuphila icubungula e Dynamics yabo.

Kuyadingeka ukusho ukuthi microscopy Fluorescent ikhuthaza izindlela ezinhle kunazo Histology futhi cytochemistry, uphethwe okusheshayo.