PCR uphethwe yokungenwa yizifo ezithathelwanayo, nobukhulu bayo

zempilo yabantu incike yokuphila kwakhe okuningi njengoba 50%. Lokhu kuhlanganisa izinto ezifana siqu kwenhlanzeko, ukudla, ukuphila kwansuku zonke, kanye ukuba khona noma ukungabi imikhuba emibi. Nokho, akekho oye wakwazi ukulondoloza impilo ngokuphelele iminyaka eminingi, ngisho nokubonakala kokungqubuzana isimo eyanelisayo akukwazi yisiboniso isimo.

ukuthuthukiswa imithi

Kule ndaba, belingana ukubaluleka ekuvimbeleni izifo indima enkulu luwathathe ukuthola kusenesikhathi. Ngalesi nesandla ekwenzeni ukuthi i-biotechnology zakamuva, ukudala revolution kulo mkhakha. Lokhu ngekhompyutha kanye-magnetic resonance imaging, futhi i-PCR ukuhlola izifo kanye spectroscopy ka yenuzi resonance kazibuthe, ngisho ukwakhiwa isengezo 3-D amamodeli we kwezitho zomzimba ahlukahlukene nezitho zomzimba. Ngokuthuthukisa imithi yezesayensi akemi namanje, ukuvula for iziguli ezingeni ehluke ngokuphelele yokusebenzisa leli ukunakekelwa kwezempilo.

Ukuhambisana nesikhathi

Njengoba sekushiwo, PCR sitholakele izifo ngesikhathi nje yashaya izwe microbial futhi wabonisa amathuba amasha ekutholweni etiology yesifo ngamunye. Ngaphambi kokutholakala yayo, ososayensi wasebenzisa izindlela isimiso ngaphezulu - izitshalo, ukutshalwa, serology, njll

Yiqiniso, Amagciwane webhaktheriya titfulwa kuze kube manje, kodwa lezi Amagciwane okufana nama-virus, mycoplasma futhi chlamydia, ezibaluleke ngokukhethekile ngenxa yokuba yinkimbinkimbi ekukhuleni kwayo endaweni yokufakelwa. Ngaphezu kwalokho, ngosuku lokuqala bayizinkulungwane esimweni njalo izinto ngokuguqula izakhi, elibuye inkimbinkimbi umsebenzi lodokotela. Kodwa uphethwe i-PCR izifo kuyinto jikelele. It ikuvumela ukuba ukunquma esiyingqayizivele kunazo zonke biomaterial nganoma iyiphi gciwane - yayo isimiso sezakhi zofuzo, noma kunalokho, acid nucleic. Lokhu kwenza kube lula ukubona uhlobo lwegciwane nge 100% ngokunembile, ukuvumela ukuba ngokushesha nika ukuphathwa efanele kakhulu ngaphandle ilimaza flora ejwayelekile imithi ebulala izinhlobonhlobo zamagciwane. Ngakho, i-PCR sitholakele izifo ezitholakala ukusebenzisa kwayo ngempumelelo kwezokwelapha, ukuvumela ukuthola ukuhlonza igciwane kunoma iyiphi biopsy noma ukuhlaziywa womuntu: igazi, ematheni cerebrospinal fluid noma scrapings.

indlela

Le ndlela liqukethe umthamo amaningi of acid nucleic ku biomaterial kuze kuze kube ufuzo ephelele ube intambo noma ngisho nezinhlayiya microbial lonke. Ngakho kuqala kukho konke-ke waqala lisetshenziswe sitholakele isifo sokusha kwesibindi kohlobo viral, ezifweni ezithathelwana ngobulili,-HIV, CIP, HPV kanye nesifo sofuba. Kukhona ukuhlaziywa ahlukene - PCR ukuhlola 12 izifo, kuhlanganise ezivamile sezifo ezidluliselwa ngobulili. Ngakho, le ndlela ungakwazi ukuthola i-chlamydia, Candida, CMV, HPV, gardnerelly, herpes simplex virus, Trichomonas, Neisseria, Mycoplasma futhi Ureaplasma. Kukhona ukuhlola 5-futhi 6-ingxenye-PCR izifo. Intengo novivinyo olunjalo siphakeme ngokwanele ukuyisebenzisa kabanzi futhi njalo (ruble mayelana 2000.).