Electrophoresis amaprotheni igazi

Amaphrotheni kukhona izenzakalo ezivelele zonke kwamangqamuzana nezicubu. Basuke ezakhiwe amaketanga amino acid. Kubantu, kukhona izinhlobo ezingaphezu kuka-100-protein molecule. Bonke bona ezihlukahlukene imisebenzi. Phakathi amangqamuzana isehlane fibrinogen transferrin, immunoglobulin, ayisiza, lipoproteins,-albumin, nabanye. Kwemihlambi amaprotheni amafraktjhini ngezindlela ezihlukahlukene, kodwa ethandwa kakhulu electrophoresis kuluthola. Cabangela izici zayo ngokuningiliziwe.

Uhlolojikelele

Ubude Amaprotheni egazi yakha "amaprotheni Imininingwane". It yena, kuhlanganisa izingxenye ezifana albumins globulin. разделяет их на эти элементы. Electrophoresis amaprotheni igazi elibahlukanisile ku lezi zinto. Le ndlela amandla division ukuletha ukuhlola ezingeni elilandelayo.

ngokucacile

. Ama-molecule ukuthola icala negative noma positive, ukuthi kuncike esimeni electrophoresis wenziwa amaprotheni zegazi. On ukunyakaza kwabo kuthinta inani ophethe. Uhlamvu ukunyakaza kunqunywa futhi ukuma nosayizi yezinhlayiya ngokwabo, isisindo sabo. Elements nenkokhiso omuhle abe adsorption kunokubuthwa okungekuhle.

albumin

Basuke kubhekwe kwekulu amaprotheni molecule phakathi kwawo wonke amaqembu aphikisanayo ku serum. Inombolo ibonisa isimo amaprotheni-albumin kwezitho eziningi zangaphakathi. Njengomunye ezimqoka yezinhlayiya uba ekulondolozeni colloidal ingcindezi osmotic. Kuyasiza ukugcina uhlelo uketshezi kule egazini. Ngakho, kungenzeka ukuchaza ukuthuthukiswa izimo zokucabanga okubangelwa isifo ezifana edema yamaphaphu, ascites, kanjalonjalo.

globulin

Basuke zihlukaniswe ngamaqembu eziningana. позволяет провести их количественное разделение в лаборатории. Amaphrotheni electrophoresis ndlela ivumela ukuba afeze ukuzihlukanisa kwabo belinani lab. Phakathi imibuso eyakha lo globulin ekhishwe inyumbazane:

1. Alpha-1. Lezi zici zimumethe-alpha-1-antitrypsin, futhi thyroxine ngibopha globulin.
2. Alpha-2. Ziqukethe izingxenye ceruloplasmin, haptoglobin nabanye.
3. Beta-izakhi. Phakathi kwabo kukhona izingxenye umphelelisi transferrin, beta-Esilula.
4. Gamma ingxenye. Iqukethe immunoglobulin, ayisiza A, E, F, G, D.

с увеличением частей альфа-1 и альфа-2 указывает на начало воспалительного процесса. Electrophoresis amaprotheni kanye nezabelo anda-alpha-1 futhi i-alpha-2 ubonisa kuqala ukuvuvukala.

kuyinsakavukela

Electrophoresis amaprotheni kubonise umzimba uphilile kulandela (e g / dl):

1. Albumin 3.4-5.
2. Alpha 1 globulin - kusuka 0.1 kuya 0.3.
3. Alpha-2 - kusuka 0.6 kuya 1.
4. Beta-globulin - kusuka 0.7 kuya 1.2.
5. Gamma globulin - kusuka 0.7 kuya 1.6.
6. izinkomba Jikelele - kusuka 6.4 kuya 8.3.

Izinzuzo uphethwe

Njengoba kushiwo ngenhla, isetshenziswa kwezokwelapha izindlela ngokwahlukana ngokwanele wamaprotheni molecule ngemibandela ethile. Nokho, esivame kakhulu electrophoresis amaprotheni. фракции, содержащиеся в определенных биологических средах, могут выделяться только этим способом. Amaphrotheni amafraktjhini equkethwe ezindaweni ezithile eziphilayo kuphela zabelwe ngale ndlela. Ikakhulu, ikuvumela ukuthola paraproteins. специальный клинический способ анализа. Amaphrotheni electrophoresis - indlela ekhethekile sokuhlaziya emtholampilo. Lwenza ihlonze noma yibuphi izinguquko zomoya, okuyinto ingaba njengesihluthulelo izimpawu of pathologies ezithile. – доступный способ диагностики. Electrophoresis amaprotheni amafraktjhini - indlela ezingabizi uphethwe. Wenzé izenzo zonke laboratories. Njengoba izinzuzo undoubted kufanele okuthiwa ukunemba nejubane yokuthola imiphumela. позволяет выявить изменения: Electrophoresis ka serum amaprotheni wembula izinguquko:

1. Ngo ukwakheka amaprotheni molecule.
2. Isilinganiso ambalwa of the timphawu tesakhiwo.

Imiphumela yocwaningo

Capillary electrophoresis ivumela ukumambula ezinye izinhlobo amaprotheni. Nokho, abanye yezinhlayiya ayikwazi ukutholwa le ndlela. I sokwehlukile-albumin. Ukuze ukuhlaziywa ezijulile kwamafrakshini esetshenziswa electrophoresis. Izinga amaqembu ahlukahlukene lingalinganiswa ngenani Imininingwane amaprotheni Inkomba siphindwe isihlobo Ingxenye% ngayinye.

umehluko wemisindo nemiqondo yamagama

обязательно должен выполняться одновременно с измерением содержания иммуноглобулинов М, А и G. Варианты с большей концентрацией первых двух, которые не могут отдельно исследоваться, необходимо направить на повторный анализ. Electrophoresis amaprotheni kufanele kwenziwe kanyekanye ne nesilinganiso immunoglobulin, ayisiza M, A no G. okwahlukile emakamu ephakeme wokuqala ezimbili, esingenakubalwa ngemibuzo bengabodwa, kubalulekile ukuthumela kabusha ukuhlaziywa. Kuyadingeka ukuba ngakususi amaqembu amancane immunofixation paraproteinovyh.

emitholampilo isithombe

позволяет обнаружить начало течения патологий почек и печени, генетические деформации, формирование опухолей злокачественного характера, активацию хронических и острых инфекций. Electrophoresis amaprotheni ivumela ukuthola ukuqala ukugeleza pathologies izinso futhi isibindi, ubunzima zofuzo, ukwakheka abulalayo izimila uhlamvu kusebenze ezingalapheki kanye nezifo sibi. Ngo umkhuba, waqokomisa eziningi "syndromes", elibonisa emagama yokuhlaziya:

1. Ingxenye anda-alpha-1 futhi i-alpha-2 globulin, fibrinogen amaprotheni C-esisheshayo, kanye eziningi acute okhombisa amaprotheni kubonisa ekuqaleni inqubo acute ukuvuvukala ne ukusebenza uhlelo umphelelisi. Lapho uqhuba elula Haematology ukuhlaziywa kulesi simo uyokwambulwa ESR anda kuphela leukocytosis.
2. Ukwehlisa emakamu eliphelele-albumin kubonisa isibindi ezifweni ezimbi. ukusha kwesibindi kohlobo Okungalapheki futhi ziphathwe yisifo sokuqina zenzeka lapho inani gamma-globulin. показывает их превышение над альбуминами, необходимо незамедлительно повторить исследование и направиться на комплексное обследование. Uma amaprotheni electrophoresis libonisa ngokweqile yabo phezu-albumin, kunesidingo esiphuthumayo ukuphinda cwaningo futhi ikhanda luhlolo olunzulu.
3. A ukwanda ngokulinganisela beta-, gamma- futhi alpha-2-globulin nge ukwehla okuncane nje kwamazinga albumin kubonisa collagenoses, ukuvuvukala okungamahlalakhona, umdlavuza neoplasms imvelo kungavamile ukuba ingozi, komzimba nezinto, pathologies sokuhlasela kwamasosha omzimba.

nephrotic syndrome

Yena sitholakale lapho kulungiswa kunocwaningo olubonisa ukwanda ezingeni filtration yezinhlayiya amaprotheni we tubules kwezinso futhi proteinuria zikhethe. Lesi sakamuva inqwaba excretion-albumin, futhi inani elincane ongaphakeme yamangqamuzana isisindo globulin umchamo. Kanye Ukulandelana lesi sifo sitholakala zamagama olunzulu zishintshe enkulu iqembu alpha-2-globulin esibindini. Zinqwabelanisa oketshezini igazi. Kule ndaba, kwakheka iphethini esinjalo. Inciphisa-albumin, futhi kwandisa umthamo-alpha-2-globulin.

ngaphezu kwalokho

Obalulekile amaprotheni ukulahlekelwa Awuboshezelwe nephrotic syndrome. Bona iyamakwa Laella isifo, ukusha olubanzi, pathologies wokugaya ukudla nokunye. Lapho Ukuhlukumeza proteinogram emathunjini emagama kubonisa ukwehla we-albumin okuqukethwe kanye nokwanda kanyekanye e amaphesenti wonke amaqembu globulin. Ukulungisa izinga amaprotheni ingatholakala wokusebenza electrophoresis. Lokuthi kunilungele ukwethula izidakamizwa, izakhi amaprotheni esikhundleni. Ngo ukwehla okuphawulekayo ku-gamma-globulin uxilongwe immunodeficiency ezinzima abalufundile noma imvelo azalwa nako. Ezimweni ezinjalo, ukukhomba ephelele isithombe emtholampilo is laphakamisa sincomo ukucacisa okuqukethwe immunoglobulin M, A, G

paraproteinemia

Electrophoresis kubhekwa indlela kuphela ivumela ukuthi ukuthola. Paraproteinemia - uphawu elikulesi sihloko isimila okuqhubekayo ukukhula imvelo ingozi nesibulalayo. Ukuthola egazini amasosha omzimba monoclonal kanye izingxenyana ubudlelwano babo bathambekele myeloma amaningi futhi leukemias ezithile. Ukuze kwamangqamuzana futhi ukusungulwa paraprotein amaprotheni ngamaketanga kunconywa ukwenza electrophoresis Ushintshe - immunofixation. Ukuze isifundo besebenzisa helium ungcwecwe ngengxube antiserum.

Izici amafraktjhini phezu ijika electrophoretic

1. Transthyretin (prealbumin). Limelela amaprotheni kwezinso. Itholakala ngaphansi albumin, i ezahlukene eyesikhashana isigamu-nokuphila. Prealbumin sibopha hormone yegilo ezokuthutha wamaprotheni kubantu Vitamin A. Okuqukethwe kwalo kusivumela ukuthi sihlaziye supply amaprotheni ka kwezicubu neuropathy. Ngezinye nokuntuleka umsoco futhi isibindi isifo kukhona ukwehla share yayo.
2. Alpha-1-lipoproteins. Zimelela iyunifomu esifundeni ngokuphelelwa umoya phakathi alpha-1-globulin futhi-albumin. Ubukhulu izingxenye zokuqala kuchazwa ezingeni elinye izakhi. Ikakhulu, i-alpha-1- antitrypsin, -fetoprotein, -microglobulin. Ngo ukuvuvukala acute waphawula ukuhwalala ebonakalayo.
3. Alpha-1 antitrypsin. kokuhlukana zaso zofuzo kuyinto kubonakala ushintsho ukunyakaza amaprotheni, ephakeme kokusebenza kwesibindi ukuhlolwa, isifo sokusha. Against ngemuva yokukhulelwa imakwe ukwehla.
4. Alpha-1-fetoprotein. Kuyinto marker pathologies hepatic ezithile nezimila azalwa naso e ukuxilongwa kokubeletha.
5. Gamma-globulin. Zone libhekene ekunqumeni izakhiwo immunoglobulin amakilasi M G.
6. Fibrinogen. He is a amaprotheni ohlelweni igazi ekunqandeni. Kutholwe phakathi gamma ne-beta-globulin agoma. Ngo ukuvuvukala acute waphawula ukwanda fibrinogen. Ngo ukwehluleka ezinzima isibindi, eqonda coagulation intravascular wembula ukwehla ezingeni layo.

immunoglobulin, ayisiza Monoclonal zitholakala kuphela khona egunjini lokuhlolwa kwezifo.