Ukudlulisa zemibungu cryopreserved. Ngo kokukhulelwa kwesibeletho

Imibhangqwana eminingi dream ukuba abazali bajabule, kodwa uphethwe ukungabi nenzalo ka oyedwa noma bobabili ophathina, negates lonke ithemba. Kulokhu, usizo eza kwesibeletho fertilization (IVF) - inqubo esisiza imibhangqwana engenanzalo ingakhetha ezintweni ukuba babe nomntwana eside elindelwe. Ukuze-ke kumelwe zilungiswe ngokucophelela, ngoba ukuqhuma hormone kwabesifazane kuholela ukuthuthukiswa ngcono amaqanda.

Ngemva kwalokhu ukuziphatha wokubhoboza wesibeletho, isb inaliti ekhethekile abazenzi nge uketshezi amaqanda engayitholi ezikuyo. Zihlukaniswa wabekwa izimo wadala ngokukhethekile bakuphi elivundisiwe futhi aqale ukuhlukanisa. I eqandisiwe ke utshalwe esibelethweni, futhi lona wesifazane elinde sokukhulelwa.

Kodwa kwenzeka ukuthi ngamapayipi yokuhlola lapho iqanda elivundisiwe, kwakhiwa imibungu omningi kunalokho okudingekile. Kulokhu, odokotela belulekwa ukuba afeze inqubo efana cryopreservation zemibungu. Zingase kudingeke uma kuqala inqubo IVF akuphumelelanga noma owesifazane esikhathini esizayo ufuna ngengane yesibili.

Kuyini cryopreservation?

Cryopreservation imibungu - yinqubo wazo ukunakekela ikamelo lazo okokuqandisa ephephile nge liquid nitrogen kokuba lokushisa 196 ngaphansi kwezingaqhwa. Kulokhu esitobhini zonke izinqubo kwamakhemikhali, okungukuthi, i-embryo eyeka asathuthuka, kodwa uma kwenzeka eneqhwa luhlala iphila.

Iba abakhulelwe ngokubasiza bakhululeke lapho IVF kusukela okokuqala abesifazane abaningi abakwazi. Lokhu kwenzeka kuphela 30-65% of amacala. A umzamo wesibili ukuphoqelela abesifazane ukuba ibhekane waphinda a wesibeletho inqubo kubuhlungu ngempela futhi ngempela kuhlasimulise ukushukuma, kanye wokubhoboza, kulandele ukwelashwa.

I eqandisiwe egcinwe ku liquid nitrogen, kungenziwa kubhekwe njengento uhlobo zokuphepha net uma kwenzeka ukwehluleka. Kuye kungase kutholakale ukuthi ukudluliswa zemibungu cryopreserved nesandla ekukhulelweni ne kakhulu amathuba efanayo uma udlulisa fresh.

Izimpawu ze-cryopreservation

Le nqubo eziyinkimbinkimbi wenziwa ezimweni lapho owesifazane:

• Ufuna ube umama ngokwesivumelwano;
• ine isifo esidluliselwa ngofuzo futhi ngaphambi umsebenzi ukufakelwa imibungu lidlule zangaphambi yenzeka uphethwe zofuzo, lapho imibungu iziguli ayeka, kuyilapho inani labantu okufanele okunempilo kudlulwe 4-6 izingcezu;
• ngesikhathi sokudlulisa umbungu yahlatshwa ukugula nezifo ezibangelwa amagciwane noma ezithathelwanayo ezingase liyochukuluza isisu nokuguqulwa noma ukuzalwa kwengane pathologies ezehlukene;
• Ufuna ukhulelwa emva kwesikhashana futhi;
• Nginalo IVF, kodwa wehluleka.

Cryopreservation zemibungu: ngobuhle nangobubi bawo

Le nqubo kunezinzuzo ezithile. Uma owesifazane oyinyumba, ke ongethemba ukuthi uzokhulelwa okwesibili. Cryopreservation ngesikhathi sokukhulelwa okuphindaphindiwe iyehlisa umthwalo phezu komzimba owesifazane, ngoba ngeke kudingeke ukuba uthathe imithi eziningi ezinkulu futhi sidlule wokubhoboza wesibeletho. Le nqubo ikuvumela ukuba ugcine ngesikhathi ngokuphindaphindiwe IVF, eqinisweni akudingeki pay kakade i-hormone okuthiwa i therapy ne Iqanda iqoqo.

Kungenzeka ukuthi kukhona kuyofika sokukhulelwa yesibili, kakhulu kwandisa njengoba iqanda is hhayi avundiswa nje, kodwa futhi waqala ukuhlanganyela ukuthi akusho ngaso sonke isikhathi kwenzeke ngesikhathi IVF. Inqubo usebenzisa imibungu cryopreserved, ayivumeli ukuthuthukisa lesi sifo ka hyperstimulation wesibeletho. Futhi, le ndlela kunikeza ithuba neminye imibhangqwana ukuba babe abazali njengoba imibungu simisiwe ingasetshenziswa njengoba abanikelayo.

Ngakho, izinzuzo cryopreservation abe impela eningi. Kodwa bonke inqubo efanayo usebenzisa imibungu cryopreserved has eyodwa ethile ebalulekile. Ihlala eqinisweni lokuthi iphesenti umbungu ukulahlekelwa lapho umnkantsha futhi incibilikiswa impela high.

kuyinto cryopreservation kanjani?

Of amaqanda avundiswa udokotela ukukhethela yekhwalithi ephezulu imibungu enempilo. Basuke-ke ibekwe ku ngamapayipi yokuhlola futhi iphathwa cryoprotectant, esivikela kuzo ekulimaleni.

tube ngalinye ezifana ibekwa microcontainers, kuyinto tube plastic okuyinto ephawulwe futhi agcinwe kulo bangase babe kuze 5 imibungu. Ngemva kwalokho, abazali ezibekwe cryobank futhi selehlile ngu okokuqandisa ingasheshi noma ultrarapid. Isitoreji Cryobank zemibungu cryopreserved Uthatha izinyanga kwaba amashumi eminyaka, kuye ngokuthi izifiso yowesifazane. Isimo esiyinhloko inqubo cryopreservation - izinga okuhle kanye nokusebenza okusezingeni eliphezulu zemibungu.

Kushiwo ngamuphi umqondo ukuthi inqubo incibilikiswa imibungu?

Imibungu abangu incibilikiswa emazingeni okushisa okuvamile. Ukuze wenze lokhu, tube lapho ukuthi akuphi, asuswe cryoprotectant bese ukuhambisa endaweni ekhethekile. Uma i-embryo ibizwa incibilikiswa ngokuphelele, it is utshalwe kabusha esibelethweni.

Ukufakelwa umbungu elandelayo cryopreservation emjikeletweni zemvelo noma inkanuko yobulili, kanye umjikelezo nge ukwelashwa kwama-hormone.

imithi kudingekile ngaphambi kokuthi ukudlulisa umbungu

Ukuze endometrium ilungele ukudluliswa, bese umbungu kahle wazinza odokotela linqume salokhu ezihlukahlukene imithi aqukethe hormone zesifazane. Ngakho-ke, uma silinga ukuphendula umbuzo yini imithi ukuphuza phambi ukudluliswa zemibungu cryopreserved.

Awu ukulungiselela ulwelwesi lwamafinyila kwesibeletho izidakamizwa progesterone, okuholela umbungu yimpumelelo uyasinda. Le mishanguzo zihlanganisa "Djufaston" futhi "Utrozhestan". Tablets "Proginova" nanesandla ukulungiswa kwesibeletho ekudlulisweni umbungu.

Kushiwo ngamuphi umqondo ukuthi ukudluliswa zemibungu incibilikiswa?

ukudluliselwa embryo ibizwa eyenziwa ngemva kokuya esikhathini kwenzeka ngemva IVF kuhlulekile. Spiking kanye imibungu ka-blastocyst esibelethweni ngokuvamile nsuku zonke defrosting imibungu.

Ukudlulisa iphinde itshalwe emva umbungu cryopreservation zenzeka umjikelezo ngokwemvelo uyavuselelwa noma umjikelezo elashwe ngokubuyiselwa kwama-hormone. Lokhu kunikeza ithemba ukuze kuthuthukiswe sokukhulelwa eside elindelwe.

ukudluliswa yi kuncike ezicini ezilandelayo:

• yobudala ivela ngowesifazane;
• uphenyo olwenziwe kahle ukwelashwa kwempilo;
• podsazhennyh inombolo zemibungu;
• inombolo yezinkinga ngesikhathi ukukhulelwa odlule.

Phakathi Nokho, umnkantsha emgodleni ohlangene umbungu ngokuvamile, ngaphambi kokuthi ukudlulisa yayo esibelethweni, hatching wenziwa, isb ezaziqoshwe igobolondo layo.

isiqalo kunokwenzeka nge ukuqanda incibilikiswa zemibungu

Kungenzeka ukuthi ngemva imibungu base simisiwe bese incibilikiswa, azizifanelekeli ngokuphelele ezokuthutha ngenxa nokubhujiswa kwabo. Kulokhu, ukudluliswa ngeke zenzeke.

Ilungiselela ulwelwesi kwesibeletho kuya iphinde itshalwe, ukuqhuba ukuhlaziya hormone izobonisa isimo salo. Uma ngasizathu simbe kwakukhona ukuphambuka kuzihlaka evamile hormone, inqubo yokudlulisa izokhanselwa ngoba ulwelwesi kwesibeletho kuyoba ungakulungele. Kulokhu, elinde umjikelezo olandelayo, lapho kabusha nokulungisa endometrium.

Ingabe cryopreservation zemibungu kungenzeka izikhathi ezingaphezu kwesisodwa?

Lokhu kungenzeka uma inombolo enkulu zemibungu eqandisiwe kusaqalwa yentuthuko, ngakho kangangokuthi cishe bonke of them incibilikiswa. Ukukhetha zezinhlobo kangcono iphinde itshalwe, futhi siyazi ukuthi kwenzekani eqandisiwe futhi. Dlulisa zemibungu ezimbili cryopreserved ikhuthaza ukukhulelwa, kodwa ezinye izici ukunciphisa umphumela yimpumelelo.

Ingabe cryopreservation ezithinta ukuthuthukiswa ingane?

Abazali sikhatsateke kakhulu ngemibiko umbuzo kanjani ukuthuthukisa ingane zemibungu cryopreserved. izifundo ezikhethekile sezenziwe ukusungula engokwengqondo, engokomzimba kwezinso obuhlakani ukuthuthukiswa yalezi zingane. Imiphumela kuhlulekile ukuveza noma yikuphi kwezinso. Inani lezingane ezineminyaka nge kwezinso azalwe imibungu cryopreserved, hhayi ningi than iphesenti izingane kwezinso zizalwe wokukhulelwa zemvelo.

Lokho okwakudingeka ukuze kukhokhwe inqubo

Abaningi abesifazane abashadile abanesifiso sokwazi: Kungakanani okungukuthi cryopreservation zemibungu? Lokho okwakudingeka ukuze kukhokhwe yonke umjikelezo, okuyinto ukusetshenziswa a eqandisiwe impahla, kuyoba izikhathi eziningana aphansi kwenani okuyinto ezophatha kabusha ngokuphelele olandelwayo IVF. Izindleko incike esingakanani amaseli izogcinwa cryostorage ukuthi wasebenzisa impahla donor, indlela okokuqandisa, inani imibungu egciniwe.

Ezweni lethu, izindleko cryopreservation kuyinto ruble 6 kuya kwezingu-30. Ukuze isitoreji zemibungu ngoba ngenyanga kuyodingeka bakhokhe ruble 1000 ngonyaka - ruble ayizigidi 10. Uma biomaterial ibekwe endaweni cryostorage ehlukile, izindleko isitoreji inyanga eyodwa kuyinto ruble 4000.

isiphetho

Ngakho, singaphetha ngokuthi cryopreservation usiza abesifazane abaningi ukukhulelwa ngemuva kwemizamo IVF kuhlulekile, futhi ngaleyo ndlela akuphazamisi impilo umntwana osanda kuzalwa. Abaningi imibhangqwana ukholelwa ukuthi le nqubo okungukuthi kakhulu usizo njengesilwane zokusiza ubuchwepheshe IVF olandelwayo, babasize okwengeziwe Insure.