Ukusabela neutralization, ngokuyisisekelo indlela futhi ngokoqobo

igama elithi "neutralization ukuphendula" ekhona kwamakhemikhali wezinto kusho inqubo yamakhemikhali lapho izinto ukuxubana izakhiwo esidi eziyisisekelo, okubangela ukulahleka wokusabela abahlanganyeli nalabo zamakhemikhali nezinye izakhiwo isici. Neutralization e-microbiology unalokho kubaluleka efanayo global imikhiqizo yayo ulahlekelwe izakhiwo oyizalayo. Kodwa-ke, kuyinto inqubo ehluke ngokuphelele nabanye ababambiqhaza futhi umphumela. Futhi izakhiwo begazi, okukhulunywa futhi ngokuyinhloko odokotela nesithakazelo futhi ososayensi - is ikhono a silwanyana sikwazi abangela izifo noma ukufa isilwane singangena.

Ngakho, kuyini na? Neutralization yokusabela - isetshenziswa uphethwe laboratory serological test, lapho mzimba sera amasosha omzimba zivimbela umsebenzi microbial, kanye kwekhabhoni ezinobuthi futhi izinto eziphila abakhuthele (enzyme).

izicelo

Ngokuvamile, le ndlela yocwaningo okubizwa ngalo igciwane, okungukuthi, izifo ezibangelwa amagciwane ezithathelwanayo. Ngaphezu kwalokho, kungenzeka ukuhlolwa sibhekiswe kokubili ukuhlonza lwegciwane, kanye namasosha omzimba nalokho.

Ngo Bacteriology le ndlela ngokuvamile esetshenziselwa ukuthola amasosha enzyme amagciwane ezifana antistreptolysin, antistafiloliziny, antistreptokinazy.

Indlela yokuhlola wenziwa

ukusabela neutralization kusekelwe ikhono omzimba - amaprotheni ethize mzimba igazi - zokuyithena antigen - ejenti angaphandle ukuthi ufake umzimba. Uma kudingekile lwegciwane bangabonwa futhi ukuhlonza ke elixutshwe ejwayelekile serum mzimba equkethe amasosha omzimba nge izinto eziphilayo azithathe. Ingxube wagcinwa i incubator esifanele futhi busuka uhlelo eziphilayo singangena. Lawa izilwane zokucwaninga (amagundane), iphuphu umbungu iseli isiko. In the ukungabikho begazi umphumela (ukugula noma ukufa sesilwane), singaphetha ngokuthi lena igciwane, a serum ejwayelekile eyayisetshenziswa. Njengoba, njengoba kuye kwashiwo, kuwubufakazi bokuthi ukusabela eyenzekile, ukushonelwa biosvoystv igciwane (ikhono ukubangela ukufa isilwane) ngenxa nokuxhumana serum amasosha omzimba futhi antigen. Ekunqumeni ezinobuthi algorithm yezenzo kuyafana, kodwa kukhona izinketho. Lapho ukuhlola yimuphi substrate eliqukethe ushevu, khona-ke exutshwe le serum ejwayelekile. Endabeni isifundo yokugcina esetshenziswa ukulawula into enobuthi. Waya ukusabela neutralization, ingxube is incubated njengoba ngesikhathi esithile futhi wethula uhlelo elungele ukwamukela okufundayo. imiphumela kokuhlaziywa nqubo okufanayo.

Zokwelapha kanye wezilwane, isetshenziswa njengophawu zokuxilonga yokuhlola igciwane neutralization ukuphendula wenziwa inqubo okuthiwa sera abhanqiwe.

Lena indlela ukuqinisekisa isifo ezibangelwa amagciwane. Ukuze enze lokhu isivivinyo isiguli evela kumuntu noma esilwaneni ukuthatha igazi serum kabili - ekuqaleni lesi sifo futhi ezinsukwini 14-21 ngemva kwalokho.

Uma ngemva kovivinyo wembula ukwanda inani amasosha igciwane ngezinye izikhathi-4 noma ngaphezulu, uphethwe angabhekwa baqinisekisa.