ELISA ukuhlola

IFA uphethwe ukutheleleka - lokhu ELISA ukuhlaziywa, okuyinto ngokuya kusetshenziswa imithi. Kusekelwe bangabonwa Isiguli omzimba athile noma antigen ukuhlonza ejenti causative.

Antigen kukhona izinto okuyinto ufaka umzimba, okubangela impendulo zokuzivikela wamasosha omzimba ngesikhathi ukutheleleka kuthathela zokukhiqiza amaprotheni ethize - omzimba.

Wonke umuntu ahlukene antigen we lwegciwane, ekuboneni kwabo, udokotela ungenza "portrait" we silwanyana okusolwayo. Amasosha omzimba futhi isebenza ngokukhetha futhi ukhiqiza amasosha igciwane ethize, futhi ngeke uxhumana antigen yezinye Amagciwane.

Mshini ngawo etholakalayo ELISA ukuhlola - okunjena senziwa eziyinkimbinkimbi antigen omzimba ezakhiwe omzimba-antigen e okunjena. Ukuphi amasosha omzimba noma antigen lokufeza ukusabela ngaleyo ndlela? Impela wamane, basuke ezivezwa izindlela zezimboni uhlobo ucwaningo ngalinye. Uhlobo ngalunye antigen esinelebuli noma omzimba athile enzyme isitshalo. Ngakho, ukwakheka lwamasosha omzimba oluvame-antigen eziyinkimbinkimbi, kuyoba njalo ukubunjwa yayo unelebuli, futhi ne ukwengeza i-ejenti Umbala, ngenxa yokubakhona ilebula, zonke izakhiwo okuholela ukushintsha umbala walo.

Lapho isampuli labetibambile ELISA ukuhlola, amaphoyisa ayaphenya ngalolo daba staining umfutho amasosha omzimba futhi antigen, ezisetshenziswa asendabeni Ihlahlubo apharathasi ekhethekile uqhuba ukubala inamba yabo.

ELISA Imiphumela

Uma antigen kutholwa unesifo noma amasosha ke, kusho ukuthini? Lokhu kusho ukuthi isiguli nakanjani has ukutheleleka. Futhi ELISA uphethwe anganika ukuthi ungubani silwanyana ethile obhekene ukuvela kwalesi antigen ngobuciko eliphezulu. Ngokuthakazelisayo, ngokungafani kuya izindlela virological futhi bacteriological ukuxilongwa, ukusebenza abampofu igciwane kungaphazamisi ukuzimisela kwakhe.

Kodwa kukhona esinye ohlangothini uhlamvu lwemali, uma ukuhlahlwa IFA atholakala omzimba isampula, okubonisa ngokucacile contact lesiguli kanye lwegciwane, lokhu kungase kungabi isiqinisekiso sokuthi micro-eziphilayo namanje abakhona emzimbeni womuntu. Phela, amasosha omzimba kungaba impela isikhathi eside ngisho nangemva kokuba alulame umzimba wesiguli. A negative yi IFA nge isheke olulodwa khona amasosha omzimba, futhi, kodwa lokhu ngesinye isikhathi ezinokwethenjelwa. Ukuze okuvela kuyo amasosha omzimba kuthatha isikhathi, yingakho i ukusazi ngokunembile isifo esikuphethe ukuze kabusha ukuhlaziya amasampula wenziwa amasonto amabili noma amathathu emva kokuqala. Futhi yingakho iningi ELISA ukuhlola izifo ezaziwayo kunengqondo, hhayi ngemuva Emasontweni ambalwa ngaphambili kuka ezintathu ukutheleleka.

Izinzuzo uphethwe immunoenzymatic kwezifo ezithathelwanayo

izindlela Ukuhlaziywa immunofermentatsii has eziningi eziwusizo, sibonga yena usuthandwa kakhulu eminyakeni yamuva.

Nge sesiye kungenzeka ukuxilonga anhlobonhlobo izifo. Ngokwesibonelo, izifo ezibangelwa amagciwane (cytomegalovirus,-HIV, i-herpes, i-hepatitis), iningi izifo, izifo ezidluliselwa ngocansi (mycoplasmosis, ugcunsula, chlamydia, ureaplasmosis), kanye isifo saseLyme, encephalitis, ezithwalwa nezinye.

Isikhathi esifushane lapho kungenzeka azokuqhubela isifundo, ikakhulukazi uma ufuna ukukhomba lwegciwane noma antigen kanye ukuze kutholakale izinga kwamasosha omzimba. Njengomthetho, lolu hlobo ukuhlaziywa kudinga omunye izinsuku ezintathu.

ELISA ukuhlola kungasetshenziswa ezimweni lapho microscopy noma ukutshala kuhilelani sobunzima obukhulu, uma kungukuthi akwenzeki.

Yena uyakwazi ukuhlola ukusebenza okuzivikela isiguli ukuba ukwethulwa lwegciwane.

Lapho usuyibekile isilinganiso amakilasi ezahlukene immunoglobulin, ayisiza, udokotela uzokwazi ukucacisa ngasiphi isigaba okwamanje isifo, ukubaluleka lolu lwazi angeke overestimated.

Zonke lezi zinzuzo wenzé ELISA ukuhlola umsizi esisemqoka wokuphila emkhakheni wamuva wezokwelapha.